Toll-like receptor 7/8 agonist stimulation rapidly skews the antibody repertoire of B cells in non-human primates.

ABSTRACT:

Introduction

Toll-like receptors 7 and 8(TLR7/8) recognize purine-rich single-stranded RNA from pathogens, triggering immune responses. Although TLR7/8 agonists are emerging as potential novel adjuvants, their impact on the adaptive B cell response, particularly antibody repertoire, remains unclear.

Methods

Six Indian rhesus macaques(IRMs) and six African green monkeys(AGMs) were stimulated with a TLR7/8 agonist. Peripheral blood samples were collected pre-stimulation and multiple timepoints within one week post-stimulation for flow cytometry and antibody repertoire sequencing.

Results

B cell activation was observed at 24 hours. Significant increases in antibody repertoire diversity were detected at 48 and 72 hours in both species; however, diversity remained elevated

SUBMITTER: Wang S 

PROVIDER: S-EPMC12497728 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature