Functional Selection of Tau Oligomerization-Inhibiting Aptamers.

ABSTRACT: Pathological hyperphosphorylation and aggregation of microtubule-associated Tau protein contribute to Alzheimer's Disease (AD) and other related tauopathies. Currently, no cure exists for Alzheimer's Disease. Aptamers offer significant potential as next-generation therapeutics in biotechnology and the treatment of neurological disorders. Traditional aptamer selection methods for Tau protein focus on binding affinity rather than interference with pathological Tau. In this study, we developed a new selection strategy to enrich DNA aptamers that bind to surviving monomeric Tau protein under conditions that would typically promote Tau aggregation. Employing this approach, we identified a set of aptamer candidates. Notably, BW1c demonstrates a high binding affinity (K_d=6.6 nM) to Tau

SUBMITTER: Wang B 

PROVIDER: S-EPMC12498309 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature