Stimuli-Responsive Luminophore Drives Mechanism Switch for Highly Efficient Electrochemiluminescence Immunosensing.

ABSTRACT: Although widely used in clinical diagnostics, the sensitivity of electrochemiluminescence (ECL) bead-based immunoassays is intrinsically limited by the reaction mechanism driving the emission of [Ru(bpy)₃]²⁺ on the bead surface. Depending mostly on the coreactant oxidation, the 'remote' pathway is hindered by the slow coreactant oxidation rate and the short half-lives of electrogenerated radicals. In this work, we synthesized a [Ru(bpy)₃]²⁺ derivative featuring a stimuli-responsive disulfide bond in its linker to the bead. Electrogenerated tri-n-propylamine (TPrA) neutral radicals reduce disulfide moieties, electrochemically inducing the release of Ru(II) labels in solution and thereby leading to an unprecedented mechanism shift toward the

SUBMITTER: Fracassa A 

PROVIDER: S-EPMC12498414 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature