Novel intranasal delivery of sihosogansan demonstrates rapid antidepressant activity via GABAergic and BDNF/TrkB pathways: identification of potential bioactive quality markers.

ABSTRACT:

Background

Sihosogansan (SHSGS) is a traditional medicine used to treat depression. However, conventional oral administration requires high doses and prolonged treatment periods. This study aimed to investigate the rapid antidepressant effects of intranasal SHSGS and to identify its Q-markers.

Methods

In zebrafish, SHSGS effects were evaluated in an MK-801-induced anxiety model using electroencephalogram (EEG) recordings. In mice, the rapid effects of intranasal versus oral SHSGS were compared through the open field and tail suspension tests. Mechanistic investigations combined computational network analysis with molecular studies of hippocampal tissue and primary neurons. Q-markers were identified through the integrative analysis of gas chromatography-mass spectrometry data

SUBMITTER: Tran KN 

PROVIDER: S-EPMC12498446 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature