Chromium-Doped Zinc Gallate Nanoparticles for Enhanced Enzyme-Linked Immunosorbent Assay Sensitivity: Optimization of Synthesis and Functionalization Strategies for Ultra-Low IgG Detection.

ABSTRACT: The use of zinc gallate nanoparticles (ZnGa₂O₄:Cr³⁺) (ZGO-NPs) presents significant potential for improving the sensitivity in enzyme-linked immunosorbent assays (ELISA). The persistent luminescence signal increase of these nanoparticles in the presence of hydrogen peroxide (H₂O₂) offers advantages for the sensitive detection of biomolecules. Herein, different conditions of ZGO synthesis have been investigated by varying the hydrothermal reaction duration (6, 12, and 24 h) and examining its impact in the presence of H₂O₂. These nanoparticles have been integrated into ELISA assays, using as target antigen IgG. The lowest limit of detection (LOD) of 0.2 pg mL^-1 is observed for ZGO-NPs prepared during 12 h

SUBMITTER: Ferjaoui Z 

PROVIDER: S-EPMC12499398 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature