Targeting Seipin to Alleviate Hepatic Steatosis in Zebrafish (Danio Rerio).

ABSTRACT: Loss of Seipin causes the absence of whole-body adipose but abnormal liver lipid deposition in patients, and liver expression of Seipin is decreased in mice fed a high-fat diet (HFD). However, the underlying mechanism of Seipin-regulated liver lipid metabolism remains mysterious. Here, experiments show that over-expression of Seipin down-regulates HFD-induced liver triglyceride (TG) accumulation and promotes zebrafish growth. Real-time PCR and immunoblotting suggest that Seipin interacts with Plin2 through its second transmembrane domain to inhibit the expression of Plin2 by promoting Plin2 ubiquitination, thereby ameliorating lipid accumulation. Co-immunoprecipitation (CoIP) experiments and Biomolecular fluorescence complementation (BiFC) analysis reveal a close interaction between Seipin

SUBMITTER: Li W 

PROVIDER: S-EPMC12499419 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature