A Molecular Recruitment Colocalization Platform for Visualizing Multi-Protein Interactions and Engineering Biomolecular Condensates in Living Cells.

ABSTRACT: Visualizing dynamic protein-protein interactions (PPIs) and phase-separated biomolecular condensates in live cells is crucial for understanding cellular processes. Here, a method is introduced for analyzing both PPIs and condensates, termed molecular recruitment colocalization (MRC), which localizes interacting partners and generates fluorescent signals at designated genomic loci. MRC enables the simultaneous visualization of multi-protein interactions and the preliminary estimation of binding affinities during PPIs. Through MRC, the study can quickly discover and validate new proteins or structural domains capable of forming condensates and can also integrate PPIs to study condensates. Furthermore, the versatility of MRC is demonstrated in phase-separated condensates research: programming

SUBMITTER: Peng L 

PROVIDER: S-EPMC12499469 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature