Controlling Release Kinetics of an Adjuvant from a Depot Improves the Efficacy of Local Immunotherapy in Metastatic Cancer.

ABSTRACT: Biomaterials can improve cancer immunotherapies by controlling their release and thereby optimizing their time-dependent engagement of the immune system. In this study, an approach is described to control the release of a potent immunostimulant-CpG oligodeoxynucleotide-from a genetically-encoded elastin-like polypeptide (ELP) depot. A CpG-binding ELP containing an oligolysine domain (ELP-Lys₁₂) is synthesized that electrostatically complexes CpG and formulate it with an excipient ELP. The ELP-CpG complex retains the thermally responsive phase behavior of the parent ELP, transitioning into a viscous depot at body temperature. Stepwise addition of excipient ELP predictably changes ELP-CpG transition temperature, depot dissolution kinetics, and retention of CpG within the depot. Mi

SUBMITTER: Milligan JJ 

PROVIDER: S-EPMC12499502 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature