Adiponectin Regulated by Indole-3-Acetic Acid in Paneth Cells Controls Renewal and Differentiation of Gut Stem Cells.

ABSTRACT: The intestinal epithelium is tightly regulated by intestinal stem cells (ISCs), but the precise mechanisms governing their differentiation remain incompletely understood. We here demonstrate that adiponectin secreted by Paneth cells (PCs) suppresses ISC renewal and differentiation via adiponectin receptor 1 (adipoR1). Genetic ablation of adiponectin in gut epithelial cells (adip^{fl/fl-Villi-Cre} mice) enhanced crypt cell renewal and differentiation. Single-cell RNA sequencing (scRNA-seq) revealed a significant increase in the enrichment of ISCs and transit-amplifying (TA) cells in adip^{fl/fl-Villi-Cre} mice compared to control adip^fl/fl mice. Furthermore, adip^{fl/fl/-Villi-Cre} mice exhibited accelerated regeneration of intestinal epithelial cells foll

SUBMITTER: Liu H 

PROVIDER: S-EPMC12499647 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature