The KASH protein UNC-83 differentially regulates kinesin-1 activity to control developmental stage-specific nuclear migration.

ABSTRACT: Nuclear migration plays a fundamental role in development, requiring precise spatiotemporal control of bidirectional movement through dynein and kinesin motors. Here, we uncover a differential isoform-dependent mechanism for developmental regulation of nuclear migration directionality. The nuclear envelope Klarsicht/ANC-1/Syne homology (KASH) protein UNC-83 in Caenorhabditis elegans exists in multiple isoforms that differentially control motor activity to achieve tissue-specific nuclear positioning. The shorter UNC-83c isoform promotes kinesin-1-dependent nuclear movement in embryonic hyp7 precursors, while longer UNC-83a/b isoforms facilitate dynein-mediated nuclear migration in larval P cells. We demonstrate that the UNC-83a-specific N-terminal domain functions as a kinesin-1 inhibitory

SUBMITTER: Gumusderelioglu S 

PROVIDER: S-EPMC12499928 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature