Chemoselective Characterization of New Extracellular Matrix Deposition in Bioengineered Tumor Tissues.

ABSTRACT: In both native and engineered tissues, the extracellular matrix (ECM) supports and regulates nearly all aspects of cellular pathophysiology, and in response, cells extensively remodel their surrounding extracellular environments through new ECM protein deposition. Understanding this intricate bi-directional cell-ECM interaction is key to tissue engineering, but it remains challenging to investigate. This is partly due to the limited sensitivity of conventional proteomics to capture low-abundance newly synthesized ECM (newsECM). This study presents a glycosylation-enabled, chemoselective strategy to label, enrich, and characterize newsECM proteins with augmented specificity and sensitivity. Applying newsECM profiling to bioengineered tumor tissues, either built upon decellularized ECM mater

SUBMITTER: Ling Z 

PROVIDER: S-EPMC12500236 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature