Isoimperatorin Reduces Synovial Inflammation and Fibrosis in Knee Osteoarthritis via the cAMP Signalling Pathway.

ABSTRACT: To explore the mechanism of pharmacological action of Isoimperatorin (ISO), a small molecule compound with anti-inflammatory properties extracted from the rhizome of Notopterygium incisum, in attenuating synovial inflammation in knee osteoarthritis (KOA). By establishing a rat model of KOA and using histopathology and molecular biology methods, we evaluated the pharmacological effect of ISO on synovitis. Synovial fluid from the knee joint was collected for transcriptomic and metabolomic analyses. Fibroblast-like synoviocytes were cultured in vitro, and calcium fluorescence imaging and mitochondrial membrane potential assays were performed to assess the effects of ISO on the cAMP signalling pathway and KOA-related synovial inflammation. Preliminary pharmacodynamic observations showed that I

SUBMITTER: Jie L 

PROVIDER: S-EPMC12500418 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature