Single-cell multi-omics and nursing follow-up prognostic modeling reveal SLFN4-mediated neutrophil dysregulation in traumatic brain injury.

ABSTRACT:

Background

Traumatic brain injury (TBI) is increasingly recognized as a systemic inflammatory disorder, with neutrophils playing a critical role in secondary injury. However, the phenotypic heterogeneity and clinical significance of neutrophil subsets in the early TBI immune landscape remain unclear, limiting their utility in nursing prognostic assessment and individualized care planning.

Methods

We performed an integrated multi-omics analysis-combining single-cell RNA sequencing (scRNA-seq), bulk transcriptomics, and proteomics-to dissect neutrophil diversity post-TBI. A distinct SLFN4⁺ neutrophil population was identified and further validated through in vitro functional assays and serum profiling in a TBI patient cohort. Clinical correlations and nursing

SUBMITTER: Tang W 

PROVIDER: S-EPMC12500558 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature