Antisense phosphorodiamidate morpholino oligomers retain activity in <i>Burkholderia cepacia</i> complex biofilm.

ABSTRACT:

Background

Members of the Burkholderia cepacia complex (Bcc) are known to cause severe pulmonary infections in immunocompromised hosts, namely individuals with cystic fibrosis (CF) and chronic granulomatous disease (CGD). Due to innate antibiotic-resistant phenotypes and the formation of protective biofilms, Bcc bacteria are difficult to eradicate from colonized lungs using traditional antibiotics. An alternative therapeutic approach involves the use of antisense molecules, specifically peptide-conjugated phosphorodiamidate morpholino oligomers (PPMOs). Previously, we found that PPMOs targeting the acyl carrier protein (AcpP) can reduce the burden of planktonic Bcc bacteria.

Methods

Antimicrobial activities of AcpP PPMOs were assessed against established biofilms prod

SUBMITTER: Mendez AR 

PROVIDER: S-EPMC12500672 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature