Engineering nitrogen-fixing microbiomes with waste-derived carbon sources: toward circular and resilient biofertilizer solution.

ABSTRACT: Microbiome engineering has emerged as a promising strategy to drive biotechnological developments across diverse fields. Microbiome-based fertilizers could significantly contribute to the gradual replacement of synthetic chemical fertilizers, potentially leading to substantial environmental and economic impacts. This study employed microbiome engineering to develop a self-assembled nitrogen-fixing microbial community utilizing carbon compounds from animal waste. This was achieved by enriching soil samples in bioreactors supplied with nitrogen via air pumping and fed with volatile fatty acids (VFAs) as the only carbon source. VFAs are the most common by-products of anaerobic waste fermentation. Results show a self-assembled community, dominated by Sinirhodobacter spp. (44.4%), Aur

SUBMITTER: Rodriguez-Romero N 

PROVIDER: S-EPMC12500719 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature