Comprehensive characterisation of age-related changes in cell subpopulations and tissue structural properties in secondary lymphoid organs.

ABSTRACT: Population aging is an escalating global phenomenon, wherein age-related alterations in the human immune system exacerbate the susceptibility to diseases including infections and autoimmune disorders. Secondary lymphoid organs (SLOs) are key locations for the execution of immunological responses by mature immune cells; however, age-related changes in SLOs remain relatively understudied. To address this gap, this study employed comprehensive approaches including single-cell RNA sequencing (scRNA-seq) data analysis, immunofluorescence staining, flow cytometry, and morphological analysis, to clarify the age-related alterations in SLOs in mice. The results demonstrated that aging caused senescent immune cells to accumulate and subpopulations to reorganize, with a decrease in the proportion of

SUBMITTER: Deng Y 

PROVIDER: S-EPMC12501032 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature