Glycan microarray analysis of <i>Candida</i>-related antibodies in human and mice sera guides biomarker discovery and vaccine development.

ABSTRACT: Invasive, high-mortality yeast infections caused by pathogenic Candida species are the most common fungal nosocomial bloodstream infections. The World Health Organization (WHO) has called for improved prevention and diagnostic strategies for fungal pathogens. Here, we demonstrate that Candida-related antibodies can be detected using glycan microarrays containing synthetic glycans to guide the discovery of oligosaccharide epitopes for diagnostics and vaccine design. Pure, synthetic mannans and β-glucans, resembling carbohydrates found on the surface of Candida spp., were employed to screen and compare sera from infected humans and mice with noninfected individuals. IgM antibodies directed toward β-glucans were observed shortly after infection, and after a

SUBMITTER: Reuber EE 

PROVIDER: S-EPMC12501125 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature