A single-cell transcriptomic study of milk cells from dairy cows with divergent lactation performance.

ABSTRACT: High-lactation cows exhibit advantages in milk yield and quality compared to low-lactation cows; however, the underlying mechanisms remain unclear. Based on the demand for high-quality milk sources in the food industry, this study used single-cell sequencing technology (scRNA-seq) on the 10 × Chromium platform to analyze the milk cells of 10 Holstein cows (5 in the high-lactation group and 5 in the low-lactation group). The seven cell types included two types of epithelial cells (epithelial and secretory epithelial cells) and five types of immune cells (neutrophils, T cells, macrophages, B cells, and dendritic cells). Further sub-clustering analysis identified three epithelial cell types and nine T-cell subsets, and their differentiation paths were depicted through pseudo temporal analysis

SUBMITTER: Ren J 

PROVIDER: S-EPMC12501235 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature