Transcriptome analysis combined with single-cell analysis identified that APOC1 influences cholesterol transport by macrophages in ccRCC.

ABSTRACT: Renal cell carcinoma (RCC) is one of the most prevalent categories of cancer worldwide, accounting for approximately 2% of cancer diagnoses and cancer deaths worldwide. various immunotherapeutic strategies have emerged as key approaches to managing advanced RCC. Bioinformatics methodologies were employed to analyze the TCGA-KIRC RNA sequencing dataset, GSE105261, GSE168845, and single-cell dataset GSE121636. Various techniques, including PCR, Western blotting, migration assays, scratch wound assays, flow cytometry, and immunofluorescence staining, were utilized to elucidate the functional characteristics of the tumor. In this study, we obtained transcriptome RNA sequencing data from the Cancer Genome Atlas (TCGA) database. Using the ESTIMATE algorithm, 249 immune-related genes were identif

SUBMITTER: Guo F 

PROVIDER: S-EPMC12501262 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature