One-step degradation of 4 classes of β-lactam using β-lactamase enzyme cocktail.

ABSTRACT: Antibiotics, particularly β-lactams, are emerging environmental contaminants that induce antibiotic-resistant bacteria or genes, which threaten public health. Enzymatic degradation is an environmentally friendly approach to reducing residual antibiotics; however, its application is limited by enzyme's narrow substrate spectrum, which cannot deal with the co-occurrence of multiple β-lactam classes in the environment. To address this issue, four representative β-lactamases were biochemically characterized and two of them (CTX-33 to VIM-1) were selected because of their broader substrate spectrum and better stability. Compared to a single enzyme that can only degrade the ring structure of three classes of β-lactam antibiotics at most, the enzyme cocktail (with a ratio of CTX-33 to VIM-1 of 4:

SUBMITTER: Lin J 

PROVIDER: S-EPMC12501275 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature