Optimizing Lentiviral Vector Production: Insights Into PiggyBac Transposase and Concatemeric Array Strategies.

ABSTRACT: Lentiviral vectors (LVVs) are essential tools in gene and cell therapy due to their ability to transduce both dividing and non-dividing cells. Conventional production by transient plasmid co-transfection is variable, costly, and difficult to scale, prompting development of stable producer cell lines. Historically, the GPRTG cell line has been generated using concatemeric-array integration, which requires high DNA input, complex workflows, and can cause genetic instability. To address these limitations, we evaluated a transposase-mediated integration strategy. Compared with the concatemeric-array method, transposase-based integration enabled faster recovery after selection with only a mild viability crisis and required substantially less DNA. This approach generated highly diverse and heter

SUBMITTER: Roscheise J 

PROVIDER: S-EPMC12501403 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature