Multiple label-free biodetection and quantitative DNA-binding assays on a nanomechanical cantilever array.

ABSTRACT: We report a microarray of cantilevers to detect multiple unlabeled biomolecules simultaneously at nanomolar concentrations within minutes. Ligand-receptor binding interactions such as DNA hybridization or protein recognition occurring on microfabricated silicon cantilevers generate nanomechanical bending, which is detected optically in situ. Differential measurements including reference cantilevers on an array of eight sensors can sequence-specifically detect unlabeled DNA targets in 80-fold excess of nonmatching DNA as a background and discriminate 3' and 5' overhangs. Our experiments suggest that the nanomechanical motion originates from predominantly steric hindrance effects and depends on the concentration of DNA molecules in solution. We show that cantilever arrays can be used to inve

SUBMITTER: McKendry R 

PROVIDER: S-EPMC125015 | biostudies-literature | 2002 Jul

REPOSITORIES: biostudies-literature