Novel prolyl endopeptidase inhibitor from <i>Myricaria germanica</i> alleviates steatohepatitis.

ABSTRACT: Prolyl endopeptidase (PREP), a serine protease, plays a critical role in the progression of hepatic steatosis and thereby contributes to metabolic dysfunction-associated fatty liver disease (MAFLD). Its inhibition has been shown to reverse disease progression. This study aimed to identify effective PREP inhibitors derived from Myricaria germanica, a deciduous shrub widely used in folk and traditional Chinese medicine, and to assess their potential therapeutic role in steatohepatitis. A bioassay-guided approach was employed to isolate PREP inhibitors from M. germanica crude extracts. The most active inhibitor was assessed through kinetic and computational studies. Moreover, its protective effects were evaluated using palmitic acid (PA) induced lipotoxicity in HepG2 cells and a

SUBMITTER: Ullah K 

PROVIDER: S-EPMC12501607 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature