Isolation of a Tissierellaceae Bacterium Exhibiting a High Reduction Potential for Insoluble Indigo Dyes.

ABSTRACT: In traditional indigo dyeing, water-insoluble indigo is anaerobically converted into soluble leuco-indigo via microbial reduction in alkaline dye suspensions, allowing its use as a fabric dye. Although various indigo-reducing bacteria have been isolated to date, culture-independent microbial community ana-lyses have suggested that bacteria belonging to uncultured clades also contribute to indigo reduction. Therefore, we aimed to isolate previously overlooked indigo-reducing bacteria using an unconventional culture method. We conducted enrichment cultures and single-colony isolation using a medium supplemented with sukumo, an indigo dye source derived from the composted leaves of indigo-containing plants, as the sole energy, carbon, and nitrogen sources. We isolated a previously uncultured

SUBMITTER: Tu Z 

PROVIDER: S-EPMC12501876 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature