Specimen Inactivation Methods for Proteomics─Comparisons of Irradiation, Chemical, and Heat Treatments on Downstream Serum Analyses.

ABSTRACT: Multiomic techniques, including proteomics, can provide novel insights for both pathogen detection and assessment of host responses to infection. Numerous studies have described the efficacy of heat inactivation, γ irradiation, or treatment with guanidinium-based chaotropic salts (e.g., TRIzol) for pathogen inactivation of biological samples to ensure biosafety and biosecurity. However, the effect of these methods on the greater serum proteome is less studied. Here, we sought to comprehensively measure the effects of four routinely used pathogen inactivation methods on the serum proteome of Rhesus macaques by characterizing the serum proteome pre-and-post inactivation treatment. Using the R-package MS-DAP for statistical benchmarking, our analysis revealed distinct changes in total peptide

SUBMITTER: Genovese BN 

PROVIDER: S-EPMC12501927 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature