Human Coronavirus-229E Hijacks Key Host-Cell RNA-Processing Complexes for Replication.

ABSTRACT: The recent rise in zoonotic coronavirus outbreaks underscores the urgency to understand virus-host interactions to develop potent antiviral therapeutics. Systems biology approaches, particularly proteomics, have been invaluable in providing a global overview of such interactions. However, these conventional approaches rely on measuring protein abundance changes that do not capture all molecular changes associated with altered regulatory pathways. In this study, we employed a high-throughput structural proteomics approach called limited proteolysis-based mass spectrometry (LiP-MS) to capture protein conformational changes, which we demonstrate are better proxies for functional alterations. We applied this tool to profile the molecular landscape of different human lung cells following human

SUBMITTER: Sarkar S 

PROVIDER: S-EPMC12501932 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature