Genome assembly and DNA methylation variation in an epimutant population of hybrid poplar clone NL895.

ABSTRACT: Epimutant populations represent important genetic resources for plant breeding and selection. However, the variation and dynamics of epigenomic modifications among epimutants are still elusive. In this study, we analyzed DNA methylation patterns at both whole-genome and allelic levels in an epimutant population of a model hybrid poplar NL895 (Populus deltoides × Populus euramericana cv. "Nanlin895"). Epimutants were generated through the application of 5-Azacytidine (5-Aza) during tissue culture. A haplotype-resolved assembly of NL895 was constructed to serve as a reference for epigenomic analysis. Compared to control plants, averaged DNA methylation levels across the entire genome were reduced in epimutants. The methylation patterns of epimutants exhibited high diversity in several aspect

SUBMITTER: He J 

PROVIDER: S-EPMC12501979 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature