Comparison of food extraction techniques and impact of nitrogen fertilization on the potential allergenicity of soybean related to birch pollen-food allergy syndrome.

ABSTRACT:

Background

Birch pollen-food allergy syndrome is triggered by cross-reactive allergens in plant-based foods. Environmental factors such as nitrogen fertilization may influence food allergenicity, but this has not been studied before.

Methods

We compared and optimized protein extraction protocols for birch-homologue foods, including apple, carrot, and soybean. Various extraction buffers and mixing methods were tested for consistency and protein yield. We applied this to a pilot study assessing potential changes in the allergenic potential of plant-based foods due to altered nitrogen availability. A greenhouse experiment was conducted in which soybean plants were subjected to different nitrogen fertilization treatments. Allergenicity was evaluated using ex vivo basophil

SUBMITTER: Verscheure P 

PROVIDER: S-EPMC12502077 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature