Osteoblast dysfunction associated with mitophagy suppression under simulated microgravity.

ABSTRACT:

Background

Bone loss is a significant health concern during spaceflight and mechanical unloading. Simulated microgravity (SMG) disrupts bone homeostasis by inhibiting osteoblast proliferation and differentiation while promoting apoptosis. Although these functional effects have been reported, the underlying mechanisms remain unclear. Mitochondrial quality control, particularly mitophagy involving the PINK1/Parkin pathway, may play a key role. This study aimed to investigate the relationship between osteogenic dysfunction and mitochondrial damage under SMG conditions and preliminarily validate the potential link using the small molecule probe icariin (ICA).

Methods

An SMG model was established using a rotary cell culture system. Cell proliferation was assessed by CCK-8 assay,

SUBMITTER: Xue J 

PROVIDER: S-EPMC12502147 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature