Development of an indirect ELISA for Duck circovirus detection based on prokaryotically expressed antigen.

ABSTRACT: Duck circovirus (DuCV) is an immunosuppressive virus that primarily causes growth retardation and immunosuppression in infected ducks, often leading to secondary infections, thereby posing a serious threat to the global duck industry. Currently, most indirect ELISA assays for DuCV rely on antibodies targeting the Cap structural protein; however, these methods cannot distinguish between antibodies generated by natural infection and those induced by vaccination. In this study, the Rep non-structural protein and truncated Cap structural protein lacking the nuclear localization signal (ΔCap) were successfully expressed using a prokaryotic Escherichia coli expression system. Two indirect ELISA methods were developed using the two proteins as coating antigens, and the method conditions were furt

SUBMITTER: Wu M 

PROVIDER: S-EPMC12502210 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature