Integrative analysis of Hub1 overexpression: driving transcriptional reprogramming and alternative splicing in Saccharomyces cerevisiae.

ABSTRACT:

Background

Hub1, a conserved ubiquitin-like protein, is essential for pre-mRNA splicing and transcriptional regulation in Saccharomyces cerevisiae. Despite its known functions, the genome-wide effects of Hub1 overexpression remain largely uncharacterized. This study investigates the transcriptomic and splicing landscape changes triggered by Hub1 overexpression using an integrative bioinformatic approach.

Results

We analyzed RNA-seq data from the GSE84215 dataset, employing differential expression, alternative splicing, functional enrichment, and network-based methods. DESeq2 identified 3,915 differentially expressed genes (DEGs; 1,964 upregulated, 1,951 downregulated, padj < 0.05), demonstrating extensive transcriptional reprogramming. Principal component analysis revealed t

SUBMITTER: Billah NMA 

PROVIDER: S-EPMC12502333 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature