Whole genome sequencing for tuberculosis disease species identification, lineage determination, and drug resistance detection in Kashgar prefecture, China.

ABSTRACT:

Background

We aimed to use whole genome sequencing (WGS) to determine species and lineage composition and drug resistance profile in a high tuberculosis (TB)-burden region of China.

Methods

We conducted WGS to 1791 acid-fast staining positive and culture-positive isolates collected from Kashgar prefecture in 2020. Bioinformatic analysis was applied to confirm species, lineage and drug resistant-related mutations. The drug susceptibility testing was performed on confirmed Mycobacterium tuberculosis complex (MTBC) isolates. We determined the accuracy of WGS prediction by comparing with phenotypes.

Results

95.03% (1702/1791) were identified MTBC, 3.18% (57/1791) were nontuberculous mycobacteria (NTM), 0.61% (11/1791) were nocardia, 0.89% (16/1791) were gordonia and 0.056

SUBMITTER: Liu D 

PROVIDER: S-EPMC12502365 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature