Computational investigation of the sequence context of arginine/glycine-rich motifs in the human proteome.

ABSTRACT: Arginine-glycine (RG)-rich motifs are among the most prevalent RNA-binding elements within intrinsically disordered regions (IDRs) of proteins and play crucial roles in RNA metabolism, gene regulation, and the formation of membraneless organelles via liquid phase separation (LLPS). Despite their biological relevance and implication in neurological disorders and cancer, the sequence features and context dependencies that define functional RG motifs remain poorly characterized owing to their disordered nature and sequence variability. In this study, we present a computational framework to dissect the sequence and structural context of RG motifs across the human proteome. By contrasting a functionally defined positive dataset-enriched for RNA-binding and phase-separating proteins-with a negat

SUBMITTER: Schumbera E 

PROVIDER: S-EPMC12502372 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature