Overcoming phage resistance: efficacy of sequential phage-colistin therapy against carbapenem-resistant <i>Acinetobacter baumannii</i>.

ABSTRACT: Carbapenem-resistant Acinetobacter baumannii (CRAB) is a major public health threat due to high mortality and limited treatment options. Bacteriophage (phage) therapy offers a promising alternative, but its long-term efficacy is challenged by the rapid emergence of phage-resistant bacterial populations. This study evaluates phage vB_AbaSt_W16 and whether sequential phage-antibiotic therapy enhances bacterial clearance and suppresses resistance. The optimal multiplicity of infection (MOI) for vB_AbaSt_W16 was determined across various CRAB strains. PAS was assessed in vitro using meropenem, colistin, tigecycline, rifampin, and ampicillin/sulbactam. An immunocompromised BALB/c mouse model was used to evaluate the therapeutic efficacy of phage monotherapy and combination therapy

SUBMITTER: Choi Y-J 

PROVIDER: S-EPMC12502580 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature