Multi-omics insights into the metabolic reprogramming of host cells triggered by <i>Entamoeba histolytica</i> Gal/GalNAc lectin intermediate subunit.

ABSTRACT: The Gal/GalNAc lectin in ‌Entamoeba histolytica‌ is pivotal for its pathogenicity, and its intermediate subunit (Igl) is a crucial virulence factor. Here, the interference of eukaryotically expressed Igl on normal metabolic processes within Caco-2 cells was explored. Targeted metabolomic analysis detected 232 metabolites in Caco-2 cells, revealing 63 with significant alterations after Igl treatment. Notably, the lactate content significantly increased, indicating that Igl activates aerobic glycolysis. Metabolic flux analysis demonstrated that Igl could inhibit aerobic respiration and the tricarboxylic acid cycle in host cells. It significantly increased glucose intake, lactate production, and ATP generation, demonstrating its ability to induce a Warburg-like effect. Through single-c

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC12502685 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature