Mutant <i>gltS</i> alleles enable a <i>Vibrio fischeri</i> D-glutamate auxotroph to grow with lower requirements for exogenous D-glutamate.

ABSTRACT: D-glu is a key component of peptidoglycan (PG) and is essential for growth in most bacteria. To assess constraints on PG evolution and bacterial requirements for D-glu, we sought to artificially evolve PG biosynthesis, leading to either replacement of D-glu in the PG peptide or alternative pathways to D-glu incorporation. We previously found that suppression of D-glu auxotrophy in a murI racD mutant of Vibrio fischeri grown on lysogeny broth salts (LBS) medium was rare but could be accomplished by mutation of bsrF, with restoration of wild-type PG structure. Here, we selected nine additional prototrophic suppressors of the same murI racD mutant from 10¹⁰ colony-forming units (CFU) plated on LBS supplemented with ~2.7 mM D-gln. Each suppressor had a mu

SUBMITTER: Coppinger M 

PROVIDER: S-EPMC12502697 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature