Defects in energy metabolism increase the susceptibility of <i>Staphylococcus aureus</i> and its small colony variants (SCVs) to <i>Staphylococcus lugdunensis</i> and lugdunin.

ABSTRACT: Lugdunin, produced by Staphylococcus lugdunensis, exhibits potent antimicrobial activity against a broad range of Gram-positive bacteria, including Staphylococcus aureus. This study aims to elucidate the mechanisms underlying the susceptibility of S. aureus to lugdunin by screening a transposon-based mutant library generated using the lugdunin-susceptible S. aureus strain HG001. We identified four S. aureus mutants with mutations in ctaA, hemY, qoxA, and guaB, which are involved in ATP and GTP synthesis, and displayed increased susceptibility to S. lugdunensis. Further tests using synthetic lugdunin confirmed that energy generation defects make S. aureus more susceptible to lugdunin. Moreover, these mutants

SUBMITTER: Liu C-C 

PROVIDER: S-EPMC12502701 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature