Development and application of a NP-cELISA for the detection of nucleoprotein antibodies of equine influenza virus.

ABSTRACT: Equine influenza (EI), caused by the equine influenza virus (EIV), is an acute respiratory disease that has become enzootic worldwide, resulting in frequent outbreaks and substantial economic losses within the equine industry. In this study, we developed a competitive enzyme-linked immunosorbent assay (NP-cELISA) for the detection of antibodies against the EIV nucleoprotein (NP). The assay was designed by coating plates with purified monoclonal antibodies (mAbs) against the NP protein, followed by simultaneous incubation of the test serum samples and HRP-NP antigen in a competitive binding reaction. Receiver operating characteristic (ROC) curve analysis demonstrated that the assay achieved 100% sensitivity and specificity. To assess the diagnostic performance of the NP-cELISA, we evaluated

SUBMITTER: Yang Y 

PROVIDER: S-EPMC12502703 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature