Nitric oxide donor sodium nitroprusside serves as a source of iron supporting <i>Pseudomonas aeruginosa</i> growth and biofilm formation.

ABSTRACT: Biofilm dispersal agents, like nitric oxide (NO), restore antimicrobial effectiveness against biofilm infections by inducing bacteria to shift from a biofilm to a planktonic state, thereby overcoming the antimicrobial tolerance typically associated with biofilms. Sodium nitroprusside (SNP) is a widely used NO donor for investigating the molecular mechanisms underlying NO-mediated biofilm dispersal in the nosocomial pathogen Pseudomonas aeruginosa. However, the biofilm effects of SNP are variable depending on the in vitro experimental conditions, with some studies reporting enhanced growth in both planktonic and biofilm forms instead of dispersal. These discrepancies suggest that SNP affects P. aeruginosa biofilm-residing cells beyond the release of NO. In this study, w

SUBMITTER: Bertran I Forga X 

PROVIDER: S-EPMC12502759 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature