High-Resolution 3-Dimensional Micro-CT Imaging of Intervertebral Discs Using a Novel Contrast Agent.

ABSTRACT:

Background

The nucleus pulposus (NP) in the intervertebral disc (IVD) is the first structure to exhibit degenerative changes during IVD degeneration (IDD). Currently, micro-computed tomography (micro-CT) imaging of the NP is a limiting factor in detecting IDD at an early stage. While contrast-enhanced micro-CT has been investigated, an effective contrast agent for IVD has not been identified. This study investigates potassium iodide (KI) as an effective contrast agent for micro-CT-based IVD visualization across multiple animal models to study IDD.

Methods

We collected tails and spines from mice, rats, rabbits, sheep, and stained them with KI followed by micro-CT imaging. For IDD, we performed caudal annular needle puncture surgery (NPS) in age and sex-matched mice (n 

SUBMITTER: Buckles MM 

PROVIDER: S-EPMC12502901 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature