Genetic Divergence and Antibody Expression Influence the N-Glycomes of CHO-K1 and CHO-S Cells.

ABSTRACT: While Chinese hamster ovary (CHO) cells continue to be the workhorse of recombinant therapeutic protein production, decades of genetic divergence in the industrially relevant CHO-K1 and CHO-S cell lines, are likely to have resulted in differing glycosylation capabilities. Glycosylation can influence the efficacy, serum half-life, and safety of biologics, and as a critical quality attribute of glycoprotein biopharmaceuticals, it is essential to better understand how major CHO cell manufacturing platforms diverge. We used matrix-assisted laser desorption ionization-time of flight mass spectrometry to perform N-glycomic analyses comparing CHO-K1 cells, CHO-S cells and antibody-producing daughter cell lines. The results reveal that genetic divergence in these industrially relevant cell lines,

SUBMITTER: Donini R 

PROVIDER: S-EPMC12503014 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature