The Missing Link: Connecting Cultivation Conditions and Refolding Performance via Inclusion Body Biophysical Properties.

ABSTRACT: Inclusion bodies (IBs) frequently form during the expression of heterologous proteins in Escherichia coli and are therefore important in pharmaceutical production. While it is accepted that cultivation conditions affect cultivation performance, the link to refolding performance remains unexplored. This study proposes that this interplay relies, at least partially, on the inherited biophysical properties of the IBs. Using a design of experiments approach, this study systematically explored how cultivation conditions-postinduction temperature, pH, and feed rate-affect the production of IBs containing anti-desipramine single-chain variable fragment antibodies as a model therapeutic protein. Various biophysical properties of the IBs-including hydrophobicity, secondary structure, and particle s

SUBMITTER: Rudt M 

PROVIDER: S-EPMC12503015 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature