Humin oxidation drives microbial dehalogenation in oligotrophic environments.

ABSTRACT: Energy acquisition presents a fundamental constraint for microbial survival in oligotrophic environments. Although heterotrophic organohalide-respiring bacteria (OHRB) are known to perform reductive dehalogenation in organohalide-contaminated oligotrophic ecosystems, their energy metabolism remains poorly understood. Here, we report that Pseudomonas sp. CP-1, an organohalide-respiring bacterium, can directly oxidize humin from diverse oligotrophic aquifers to drive organohalide respiration. Spectroscopy, electrochemistry, and metabolic profiling demonstrated that electrons stored in phenolic hydroxyl and amino groups of humin were utilized by strain CP-1 for organohalide respiration. Mutational and chemical inhibition studies identified an extracellular electron uptake pathway involving a

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC12503164 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature