Differential expression analysis of lncRNA and mRNA in ovarian tissues of Pishan Red Sheep and Hu Sheep with distinct genotypes during estrus.

ABSTRACT: Pishan Red Sheep and Hu Sheep are sheep breeds with exceptional reproductive characteristics. To investigate the similarities and differences in the expression of reproduction-related genes between these two breeds, this study utilized transcriptome sequencing to identify differentially expressed lncRNAs and mRNAs in ovarian tissues during estrus in Hu Sheep and Pishan Red Sheep carrying FecB^B+ and FecB⁺⁺ genotypes. Furthermore, we explored their potential impacts on fertility. Transcriptome sequencing of ovarian tissues generated 204.58 Gb of clean data. Bioinformatics analysis identified 34,651 lncRNAs, with differential expression analysis revealing 1,481 differentially expressed mRNAs and 698 differentially expressed lncRNAs. Differentially expressed

SUBMITTER: Muhetaer A 

PROVIDER: S-EPMC12504091 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature