The E3 ubiquitin ligase MARCH9 alleviates pyroptosis by regulating NLPR3 ubiquitination following myocardial ischemia reperfusion.

ABSTRACT: NLRP3, a member of the NOD-like receptor family, mediates pyroptosis via inflammasome activation, contributing significantly to myocardial ischemia-reperfusion (MI/R) injury. However, the molecular mechanisms remain unclear. This study aimed to elucidate the mechanisms underlying NLRP3 inflammasome activation and its role in pyroptotic myocyte death following MI/R injury, with a particular focus on the effects of MARCH9, an E3 ubiquitin ligase that encodes a RING finger domain. In our in vivo experiments, we established an MI/R injury model by ligating the anterior descending branch of the coronary artery in mice. Cardiac tissues were analyzed using various methods, including proteomic analysis, TTC and Evan's blue dual-dye staining, immunohistochemical staining (F4/80, CD11b and Ly6G), an

SUBMITTER: Lu H 

PROVIDER: S-EPMC12504159 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature