Mechanistic insights into miR-584-5p-mediated Inhibition of PDLSCs osteogenic differentiation through H2AFZ upregulation and RUNX2 suppression.

ABSTRACT: Periodontal ligament stem cells (PDLSCs) hold promise for bone regeneration, but their osteogenic differentiation is tightly regulated by various molecular mechanisms. MicroRNAs are key regulators of this process, and miR-584-5p has been identified as a potential modulator of osteogenesis. In this study, we investigated the role of miR-584-5p in the osteogenic differentiation of PDLSCs. Our findings show that overexpression of miR-584-5p inhibits osteogenic differentiation in vitro, as evidenced by reduced alkaline phosphatase activity, diminished mineralized nodule formation, and decreased expression of osteogenic markers, including ALPL, SP7, and RUNX2. In animal models, suppression of miR-584-5p enhances bone formation in both ectopic bone formation and rat calvarial defect models. Mech

SUBMITTER: Wang C 

PROVIDER: S-EPMC12504162 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature