Comparative analysis of 3 qPCR primer-probe sets for the detection of equid alphaherpesvirus 1.

ABSTRACT: With the revision of the World Organisation for Animal Health (WOAH) Terrestrial Manual on equine rhinopneumonitis in 2024, 3 recommended qPCR primer-probe sets were added for the detection of equid alphaherpesvirus 1 (EqAHV1; formerly equine herpesvirus 1 [EHV1]; family Orthoherpesviridae, taxon species Varicellovirus equidalpha1), also known as equine abortion virus. We compared the sensitivity and specificity of the 3 qPCR primer-probe sets to determine the most reliable set. Sets gB1H and gB1P, which target the glycoprotein B (gB) gene of EqAHV1, detected all 10 copies and even lower copy numbers. In contrast, set gC1 (ISO 17025-accredited method used at the WOAH reference laboratory), which targets the glycoprotein C (gC) gene, failed to detect ≤10 copies o

SUBMITTER: Kambayashi Y 

PROVIDER: S-EPMC12504209 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature