Revealing the essential role of the lid in mclPHA intracellular depolymerase from Pseudomonas putida KT2440.

ABSTRACT: Polyhydroxyalkanoates (PHAs) are microbial polyesters that serve as intracellular carbon reserves and represent promising biodegradable alternatives to conventional plastics. However, their large-scale application requires not only cost-effective production but also efficient strategies for recovery and recycling. Unlike short-chain-length PHAs, which are widely degraded by diverse enzymes, the intracellular degradation of medium-chain-length PHAs (mclPHAs) appears to be a genus-specific trait of Pseudomonas. In this context, the PhaZKT depolymerase from Pseudomonas putida KT2440 is considered a model enzyme for intracellular mclPHA mobilization; it is highly substrate-specific, acting almost exclusively on mclPHAs, and consists of an α/β-hydrolase fold with a lid domain, similar to lipase

SUBMITTER: de Eugenio LI 

PROVIDER: S-EPMC12504323 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature