The dynamic role of TRIM8, a novel ciliary protein, during various stages of mitosis.

ABSTRACT: TRIM8 is an E3 ubiquitin ligase that functions as both a tumour suppressor and an oncoprotein. Earlier, we reported that TRIM8 interacts with key regulators of mitotic spindle assembly, and that TRIM8 knockdown results in mitotic delay and aneuploidy. In this study, we implemented an omics strategy with differential transcriptomic (single-cell RNA sequencing or scRNA-seq), translatomic (polysome profiling with RNA-seq), and proteomic (LC-MS/MS) approaches to elucidate the involvement of TRIM8 in different levels (transcription, translation, post-translation) and stages (G0/G1, S, G2/M) of mitotic cell cycle regulation and progression. With the aid of differential transcriptomic and proteomic approaches, we show that depletion of TRIM8 perturbs the canonical 'Cell Cycle Control of Chromosom

SUBMITTER: Bhaduri U 

PROVIDER: S-EPMC12504472 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature