Genomic sequencing combined with marker-assisted breeding effectively eliminates potential linkage drag of a target gene: a case study in tobacco.

ABSTRACT: Linkage drag frequently impedes the utilization of beneficial genes from wild species in crop improvement. The N gene from Nicotiana glutinosa confers strong resistance to tobacco mosaic virus (TMV) but introduces linkage drag when introgressed into cultivated tobacco (Nicotiana tabacum). To address this issue, we sequenced the TMV-resistant flue-cured tobacco line 0970A and carried out comparative genomic analysis. Additionally, we used molecular markers to screen a BC₄F₁ population derived from the cross between 0970A and an elite flue-cured tobacco variety CB-1 (recurrent parent). As a result of sequencing 0970A, the N gene was located at the end of chromosome Nt11. The comparative genomic analysis showed that 0970A inherited approximate

SUBMITTER: Gao W 

PROVIDER: S-EPMC12504484 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature